Coding
Part:BBa_K4765014:Design
Designed by: Siliang Zhan Group: iGEM23_Fudan (2023-08-07)
MysH codon optimized
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 23
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We have codon-optimized the original sequence based on E. coli K12 to enable its expression in Escherichia coli.
Source
synthetic